aptt reagent platelin ls  (Technika Inc)

Journal: Clinical and Applied Thrombosis/Hemostasis

Article Title: An Evaluation of Hemostatic Abnormalities in Patients With Hemophilia According to the Activated Partial Thromboplastin Time Waveform

doi: 10.1177/1076029618757344

Figure Lengend Snippet: Activated partial thromboplastin time (APTT) waveform. a indicates acceleration; v, velocity; ½ f , ½ fibrin formation; 1, peak time; 2, height; 3, width; 1a, peak time of acceleration; 1v, peak time of velocity; 1 1/2 f, peak time of ½ height of fibrin formation; 2a 1 , height of acceleration peak 1; 2a 2 , height of acceleration peak 2; 2v, height of velocity; 2 1/2 f, height of ½ fibrin formation; 3a 1 , width of acceleration peak 1; 3a 2 , width of acceleration peak 2; 3v, width of velocity, bp, biphasic waveform.

Article Snippet: In previous reports, an abnormal biphasic curve of the APTT waveform was reported to be associated with the early detection of disseminated intravascular coagulation , using Platelin LS APTT reagent with the MDA II (Organon Teknika, Cambridge, United Kingdom) analyzer.

Techniques:

Journal: Clinical and Applied Thrombosis/Hemostasis

Article Title: An Evaluation of Hemostatic Abnormalities in Patients With Hemophilia According to the Activated Partial Thromboplastin Time Waveform

doi: 10.1177/1076029618757344

Figure Lengend Snippet: Activated partial thromboplastin time (APTT) waveform in a patient with hemophilia without inhibitor (A), with inhibitor (B), a patient positive for LA (C), and a patient treated with warfarin (D).

Article Snippet: In previous reports, an abnormal biphasic curve of the APTT waveform was reported to be associated with the early detection of disseminated intravascular coagulation , using Platelin LS APTT reagent with the MDA II (Organon Teknika, Cambridge, United Kingdom) analyzer.

Techniques:

Journal: Clinical and Applied Thrombosis/Hemostasis

Article Title: An Evaluation of Hemostatic Abnormalities in Patients With Hemophilia According to the Activated Partial Thromboplastin Time Waveform

doi: 10.1177/1076029618757344

Figure Lengend Snippet: Relationship Between FVIII Concentration and Parameters of APTT Waveform.

Article Snippet: In previous reports, an abnormal biphasic curve of the APTT waveform was reported to be associated with the early detection of disseminated intravascular coagulation , using Platelin LS APTT reagent with the MDA II (Organon Teknika, Cambridge, United Kingdom) analyzer.

Techniques: Concentration Assay

Journal: Clinical and Applied Thrombosis/Hemostasis

Article Title: An Evaluation of Hemostatic Abnormalities in Patients With Hemophilia According to the Activated Partial Thromboplastin Time Waveform

doi: 10.1177/1076029618757344

Figure Lengend Snippet: Parameters of APTT Waveform in Patients With Hemophilia With or Without Inhibitor, Those With VWD, and Healthy Volunteers.

Article Snippet: In previous reports, an abnormal biphasic curve of the APTT waveform was reported to be associated with the early detection of disseminated intravascular coagulation , using Platelin LS APTT reagent with the MDA II (Organon Teknika, Cambridge, United Kingdom) analyzer.

Techniques:

Journal: Clinical and Applied Thrombosis/Hemostasis

Article Title: An Evaluation of Hemostatic Abnormalities in Patients With Hemophilia According to the Activated Partial Thromboplastin Time Waveform

doi: 10.1177/1076029618757344

Figure Lengend Snippet: Parameters of APTT Waveform in Patients With Hemophilia With or Without Inhibitor, Those With VWD, and Patients With LA.

Article Snippet: In previous reports, an abnormal biphasic curve of the APTT waveform was reported to be associated with the early detection of disseminated intravascular coagulation , using Platelin LS APTT reagent with the MDA II (Organon Teknika, Cambridge, United Kingdom) analyzer.

Techniques:

Journal: Clinical and Applied Thrombosis/Hemostasis

Article Title: An Evaluation of Hemostatic Abnormalities in Patients With Hemophilia According to the Activated Partial Thromboplastin Time Waveform

doi: 10.1177/1076029618757344

Figure Lengend Snippet: Parameters of APTT Waveform in Patients With Hemophilia With or Without Inhibitor, Those With VWD, and Patients Treated With Warfarin.

Article Snippet: In previous reports, an abnormal biphasic curve of the APTT waveform was reported to be associated with the early detection of disseminated intravascular coagulation , using Platelin LS APTT reagent with the MDA II (Organon Teknika, Cambridge, United Kingdom) analyzer.

Techniques:

Journal: Clinical and Applied Thrombosis/Hemostasis

Article Title: An Evaluation of Hemostatic Abnormalities in Patients With Hemophilia According to the Activated Partial Thromboplastin Time Waveform

doi: 10.1177/1076029618757344

Figure Lengend Snippet: Parameters of Aptt Waveform Between Biphasic Pattern Positive and Negative of Patients With Hemophilia, and Those With VWD.

Article Snippet: In previous reports, an abnormal biphasic curve of the APTT waveform was reported to be associated with the early detection of disseminated intravascular coagulation , using Platelin LS APTT reagent with the MDA II (Organon Teknika, Cambridge, United Kingdom) analyzer.

Techniques:

Journal: Journal of thrombosis and haemostasis : JTH

Article Title: Improved hemostasis in hemophilia mice by means of an engineered factor Va mutant

doi: 10.1111/jth.12489

Figure Lengend Snippet: Correction of clotting times of factor V (FV)-deficient plasma by superFVa. (A) Activated partial thromboplastin time (APTT) and (B) prothrombin time (PT) clotting times were determined in FV-deficient plasma after reconstitution with FVa or superFVa (0.5 nmol L−1). Normal plasma served as control (n = 4). Error bars represent SEM (*all P < 0.0001).

Article Snippet: For the APTT assay, plasma (50 μL) was mixed with 50 μL of APTT reagent (Platelin LS; Organon Technika, Durham, NC, USA) and incubated at 37 °C for 3 min. Then 2 μL of FVa (final concentration 0.5 n nmol L −1 ) was added, followed by 50 μL of 25 mmol L −1 CaCl 2 in HBS, 0.5% BSA.

Techniques: Coagulation, Clinical Proteomics, Control

Journal: Pathophysiology of Haemostasis and Thrombosis

Article Title: Factor V Is an Anticoagulant Cofactor for Activated Protein C during Inactivation of Factor Va

doi: 10.1159/000315141

Figure Lengend Snippet: Control experiments for FVIII sensitivity of the dilute prothrombin time clotting assay. a Pooled normal human plasma was incubated in the absence or presence of 1 μg/ml BO2C11 for 2 h at 37°C and then used in the APTT assay and dilute prothrombin time clotting assay. n = 4. Error bars represent the SEM. b FV-deficient plasma was incubated in the absence (▴, ▵) or presence (•, ○) of 20 μg/ml BO2C11 for 80 min at 37°C and then used in FV titrations, in the absence (▵, ○) or presence (▴, •) of APC. Inset: APCsr. Final concentration of BO2C11 was 1.3 μg/ml. n = 1. c M662C/D1828C-FVIII was titrated in FVIII-deficient plasma and clotting time was measured in the absence (⋄) or presence (♦) of APC. n = 1. d M662C/D1828C-FVIII was titrated in FV-deficient plasma and clotting time was measured in the absence (⋄) or presence (♦) of APC. The FVIII concentration for each data point represents total FVIII present in the final reaction, which included added FVIII plus 0.067 U/ml FVIII present in 15-fold diluted FV-deficient plasma. n = 1.

Article Snippet: Platelin LS activated partial thromboplastin time (APTT) reagent was from Trinity Biotech LPC (Bray, Wicklow, Ireland).

Techniques: Control, Coagulation, Clinical Proteomics, Incubation, Concentration Assay